The effects of proliferation and differentiation on adipocyte 3T3-L1 by prescriptions and herbs of Taeyang-In and Taeum-In
Su-Beom Kim
Dept. of Oriental Medicine,
Graduate School, Kyung Hee University
Seoul, Korea
(directed by prof. Il-Byung Song, O.M.D., Ph.D.)

In order to know the effect of proliferation and differentiation on edipocyte 3T3-L1 by prescriptions and herbs, Taeyang-In()'s Okapijangcheok- tang(ʥ)Mihudeungsikjang-tang( )Acanthopanacis Cortex( ʥ )Phragmitis Rhi- zoma() and Taeum-In()'s Taeumjowi-tang ()Cheongsimyonja-tang()Cheongpaesagan-tang()Galkeunbupyong-tang(ݩ) Coicis Semen()Rhei Undulati Rhizoma()Mori Cortex()Ulmi Cortex()Holotrichia Vermiculus( )Kalopanaxii Cortex() Ephedrae Herba(ث)Imperatae Rhizoma(), were used and had some effects.

1. The proliferation effect of edipocyte

1) At the Taeyang-In()'s prescriptions and herbs, Okapijangcheok-tang (ʥ)Mihudeungsikjang-tang( )Acanthopanacis Cortex(ʥ) have a control effect at the boiling water-extract and ethyl alcohol-extract. Phragmitis Rhizoma() have a control effect at the ethyl alcohol-extract.

2) At the Taeyang-In()'s prescriptions and herbs, Teum jowi-tang()Cheongsimyonja-tang()Cheongpaesagan-tang()Galkeunbupyong-tang(ݩ) have a control effect at the boiling water-extract and ethyl alcohol-extract. Coicis Semen()Rhei Undulati Rhizoma()Mori Cortex()Ulmi Cortex()Kalopanaxii Cortex()Ephedrae Herba(ث) of the boiling water-extract., Holotrichia Vermiculus( )Kalopanaxii Cortex() of ethyl alcohol-extract have a control effect on edipocytes. Rhei Undulati Rhizoma()Ulmi Cortex()Ephedrae Herba(ث) of high-density have a cyto-toxicity.

2. The differentiation effect of edipocyte

1) At the Taeyang-In()'s prescriptions and herbs during the natural differentiation, Phragmitis Rhizoma() of the boiling water-extract, Okapijangchek-tang(ʥ)Acanthopanacis Cortex(ʥ) of the ethyl alcohol-extract have a cyto-toxicity on the first-differentiation.

2) At the Taeum-In()'s prescriptions and herbs during the natural differentiation, Ulmi Cortex()Kalopanaxii Cortex() of the boiling water-extract have a cyto-toxicity on the first-differentiation. Cheongsimyonja-tang()Ephedrae Herba(ث) of ethyl alcohol-extract have a control effect on the redifferentiation.

3) At the Taeyang-In()'s prescriptions and herbs on the first-differentiation during the induced differentiation, Acanthopanacis Cortex(ʥ) of ethyl alcohol-extract has a control effect. Okapijangchek-tang(ʥ)Acanthopanacis Cortex(ʥ)Phragmitis Rhizoma() of the boiling water-extract have a cyto-toxicity.

4) At the Taeum-In()'s prescriptions and herbs on the first-differentiation during the induced differentiation, Coicis Semen()Ephedrae Herba(ث)Imperatae Rhizoma() of the boiling water-extract and Ephedrae Herba(ث) of the ethyl alcohol-extract have a control effect. Kalopanaxii Cortex() of the boiling water-extract and the ethyl alcohol-extract has a cyto-toxicity.

Considering this result, the Taeyang-In()Taeum-In()'s prescriptions and herbs have a control effect on edipocytes during the proliferation. Acanthopanacis Cortex(ʥ), Coicis Semen()Ephedrae Herba(ث)Imperatae Rhizoma() have a control effect on edipocytes during the induced differentiation.
In the future, for treating a obesity need a vivo assay and hope this study to help to know the mechanisms of obesity.